ITS sequences variation and phylogenetic analysis on 31 geographical populations of Notopterygium incisum / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 3748-3753, 2015.
Artigo
em Chinês
| WPRIM
| ID: wpr-237737
ABSTRACT
In this study, 31 Notopterygium incisum populations were analyzed using ITS sequences to investigate the genetic structure. The results showed that the ITS region ranged in size from 634 to 635 bp and base composition was with high G + C content of 57.8%. Thirty-one polymorphic sites were detected from 402 sequences of 31 populations of N. incisum, and the proportion of polymorphic sites was 4.88%, in which parsimony informative sites were up to 12. And 31 haplotypes were identified based on these polymorphic sites. Molecular variance analysis (AMOVA) indicated that high genetic differentiation (57%) existed among population, and gene flow was low (N(m) = 0.38) among populations. Phylogenetic relationships of 31 haplotypes were analyzed using NJ method with N. forbesiias an out-group. Phylogenetic analysis showed that 31 haplotypes from different populations mixed together and did not form distinct geographically separated clades.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Filogenia
/
Variação Genética
/
Dados de Sequência Molecular
/
Sequência de Bases
/
China
/
Classificação
/
Apiaceae
/
DNA Intergênico
/
Fluxo Gênico
/
Genética
País/Região como assunto:
Ásia
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2015
Tipo de documento:
Artigo
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