Experimental study of relationship of bile composition imbalance with bile duct injury / 中华外科杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the change of bile composition and its role in bile duct injury after orthotopic liver transplantation (OLT).</p><p><b>METHODS</b>Rats were randomly divided into 3 groups group A (sham surgery), group B (OLT with 1 h cold preservation), group C (OLT with 12 h cold preservation). The arterialized rat liver transplantation model with biliary extra-drainage was used in group B and C. Animals were sacrificed at posttransplant 1, 3, 5, 7, 10 and 14 day. Routine bile chemistry and pathological assays were performed.</p><p><b>RESULTS</b>Cold preservation/reperfusion injury (CPRI) could repress the secretion of bile salts and phospholipid. However, in contrast with a rapid increase of bile salt secretion, the biliary secretion of phospholipid recovered more slowly, leading to an abnormal high bile salts/phospholipid ratio early after transplantation. Further analysis suggested that the secretion of bile salts correlated strongly with biochemical and histopathological signs of bile duct injury.</p><p><b>CONCLUSIONS</b>CPRI can lead to great changes of graft bile composition, which plays a role in the pathogenesis of bile duct injury following liver transplantation.</p>