Real-time fluorescent PCR for screening AZFc/DAZ microdeletions on the Y chromosome in male infertility patients / 中华男科学杂志
National Journal of Andrology
;
(12): 436-442, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-238003
ABSTRACT
<p><b>OBJECTIVE</b>To develop a real-time fluorescent PCR protocol suitable for the routine screening of AZFc/DAZ microdeletions on the Y chromosome in azoospermic and oligozoospermic male infertility patients.</p><p><b>METHODS</b>A set of real-time fluorescent PCR was established. Eighty-seven azoospermic and ligozoospermic patients undergoing ICSI in the IVF center and 30 azoospermic men undergoing testicular biopsy in the clinic of urology surgery were screened for AZFc/DAZ microdeletions of Y chromosome.</p><p><b>RESULTS</b>Eleven cases (9.4%) of AZFc/DAZ microdeletions were found in 117 cases of azoospermic and oligozoospermic patients by screening of realtime fluorescent PCR. Four cases (6.6%) were found in 61 oligozoospermic patients, and 7 cases (12.5%) were found in 56 azoospermic patients.</p><p><b>CONCLUSION</b>The real-time fluorescent PCR protocol presented in this study is an easy and reliable method for detection of AZFc/DAZ microdeletions on the Y chromosome, which yields identical results to those of the multiplex PCR.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Reação em Cadeia da Polimerase
/
Deleção Cromossômica
/
Proteínas de Ligação a RNA
/
Cromossomos Humanos Y
/
Fluorescência
/
Proteína 1 Suprimida em Azoospermia
/
Genética
/
Infertilidade Masculina
/
Métodos
Tipo de estudo:
Estudo diagnóstico
/
Guia de Prática Clínica
/
Estudo de rastreamento
Limite:
Humanos
/
Masculino
Idioma:
Chinês
Revista:
National Journal of Andrology
Ano de publicação:
2003
Tipo de documento:
Artigo
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