Evaluation of Behavior and Expression of Receptor Activator of Nuclear Factor-Kappa B Ligand in Dorsal Root Ganglia after Sciatic Nerve Compression and Application of Nucleus Pulposus in Rats
Asian Spine Journal
;
: 557-564, 2014.
Artigo
em Inglês
| WPRIM
| ID: wpr-23811
ABSTRACT
STUDY DESIGN:
Experimental animal study.PURPOSE:
To evaluate pain-related behavior and changes in nuclear factor-kappa B (NF-kB), receptor activator of NF-kB (RANK), and ligand (RANKL) in dorsal root ganglia (DRG) after combined sciatic nerve compression and nucleus pulposus (NP) application in rats. OVERVIEW OF LITERATURE The pathological mechanisms underlying pain from lumbar-disc herniation have not been fully elucidated. RANKL are transcriptional regulators of inflammatory cytokines. Our aim was to evaluate pain-related behavior and RANKL expression in DRG after sciatic-nerve compression and application of NP in rats.METHODS:
Mechanical hyperalgesia and RANKL expression were assessed in three groups of rats NP+sciatic nerve compression (2 seconds), sham-operated, and controls (n=20 each). Mechanical hyperalgesia was measured every other day for 3 weeks using von Frey filaments. RANKL expression in L5 DRGs was examined at five and ten days after surgery using immunohistochemistry.RESULTS:
Mechanical hyperalgesia was observed over the 12-day observation period in the NP+nerve compression group, but not in the control and sham-operated animal groups (p<0.05). RANKL immunoreactivity was seen in the nuclei of L5 DRG neurons, and its expression was significantly upregulated in NP+nerve compression rats compared with control and sham-operated rats (p<0.01).CONCLUSIONS:
The exposure of sciatic nerves to mechanical compression and NP produces pain-related behavior and up-regulation of RANKL in DRG neurons. RANKL may play an important role in mediating pain after sciatic nerve injury with exposure to NP.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Nervo Isquiático
/
Imuno-Histoquímica
/
Regulação para Cima
/
Citocinas
/
NF-kappa B
/
Negociação
/
Grupos Diagnósticos Relacionados
/
Ligante RANK
/
Gânglios Espinais
/
Hiperalgesia
Limite:
Animais
Idioma:
Inglês
Revista:
Asian Spine Journal
Ano de publicação:
2014
Tipo de documento:
Artigo
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