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Measuring rupture forces of P-selectin/PSGL-1 bonds using an optical trap assay / 生物医学工程学杂志
Journal of Biomedical Engineering ; (6): 961-965, 2005.
Artigo em Chinês | WPRIM | ID: wpr-238301
ABSTRACT
Selectin/ligand interaction plays an important role in such biological processes as inflammatory reaction, tumor metastasis, etc. External forces affect dissociation of receptor-ligand bonds. A novel approach, upon optical trap technique, was developed in this study to investigate the dissociation of P-selectin/PSGL-1 (P-Selectin Glycoprotein Ligand 1) bindings. Stiffness of optical trap was calibrated with laser power using a viscous drag method. While P-selectin and PSGL-1 molecules were functionally coated on surfaces of glass beads, respectively, the dissociation of interacting molecule bond was studied by measuring the rupture force distribution. It was found that most probable rupture force increased with loading rate at < 25 pN/s. These results complemented and validated the current theory at low loading rates.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Ligação Proteica / Glicoproteínas de Membrana / Química / Estrutura Terciária de Proteína / Óptica e Fotônica Limite: Humanos Idioma: Chinês Revista: Journal of Biomedical Engineering Ano de publicação: 2005 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Ligação Proteica / Glicoproteínas de Membrana / Química / Estrutura Terciária de Proteína / Óptica e Fotônica Limite: Humanos Idioma: Chinês Revista: Journal of Biomedical Engineering Ano de publicação: 2005 Tipo de documento: Artigo