Fosinopril but not metoprolol attenuated the increased matrix metallo-proteinases 9 expression at mRNA and protein levels of human umbilical vein endothelial cells exposed to oscillatory flow / 中华心血管病杂志
Chinese Journal of Cardiology
;
(12): 1113-1116, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-238474
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of fosinopril and metoprolol on metalloproteinases 9 (MMP9) expression of human umbilical vein endothelial cells (HUVECs) stimulated by oscillatory flow.</p><p><b>METHODS</b>HUVECs were exposed to steady laminar flow or oscillatory flow, laminar flow or oscillatory flow plus various concentrations (1 x 10(-7) mol/L, 1 x 10(-5) mol/L) of fosinopril and metoprolol for 4 and 24 hours. MMP9 mRNA and protein expressions of HUVECs were determined by RT-PCR and Western blot, respectively.</p><p><b>RESULTS</b>MMP9 expression at mRNA and protein levels were significantly increased in HUVECs exposed to oscillatory flow than that to laminar flow and these could be down-regulated by coincubation with fosinopril (1 x 10(-7) mol/L, 1 x 10(-5) mol/L, P < 0.01, P < 0.05, respectively) but not by co-incubation with metoprolol.</p><p><b>CONCLUSION</b>Fosinopril can attenuate the increased MMP9 expression at mRNA and protein levels of HUVECs exposed to oscillatory flow.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Estresse Mecânico
/
Veias Umbilicais
/
RNA Mensageiro
/
Endotélio Vascular
/
Células Cultivadas
/
Fosinopril
/
Metaloproteinase 9 da Matriz
/
Biologia Celular
/
Células Endoteliais
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Cardiology
Ano de publicação:
2006
Tipo de documento:
Artigo
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