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Observation of functional remodeling of Ca2+-activated Cl- channel in pacing-induced canine failing heart / 中华心血管病杂志
Chinese Journal of Cardiology ; (12): 797-800, 2006.
Artigo em Chinês | WPRIM | ID: wpr-238515
ABSTRACT
<p><b>OBJECTIVE</b>To study whether Ca(2+)-activated Cl(-) current (I(to2)) contributes to the functional remodeling of the failing heart.</p><p><b>METHODS</b>The cardiac myocytes were isolated enzymatically from rapidly pacing-induced failing canine hearts (HF) at room temperature. Patch-Clamp whole cell recording technique was employed to record the I(to2). The Cl(-) transport blocker 4, 4'-diisothiocyanostilbene-2, 2'-disulfonic acid (DIDS, 200 micromol) was used to isolated the I(to2). The relations of I(to2) to L-type Ca(2+) current (I(Ca-L)) and to the membrane voltage under the constant intracellular [Ca(2+)]i were evaluated in HF and the normal hearts.</p><p><b>RESULTS</b>We found that the current density of I(to2) was significantly decreased in HF cells compared with the controls. At membrane voltage of 20 mV, for example, the I(to2) density was (3.02 +/- 0.54) pA/pF in control cells (n = 7) vs. (1.31 +/- 0.25) pA/pF in HF (n = 8) cells, P < 0.05. While the averaged I(Ca-L) density did not show difference between two groups. The time constant of current decay of I(to2) was similar in both types of cells. However, in intracellular Ca(2+) clamped mode with 100 micromol [Ca(2+)]i, I(to2) density was increased significantly in HF cells at membrane voltage of +30 mV or higher.</p><p><b>CONCLUSIONS</b>Our results suggest that the decrease of I(to2) density may contribute to the prolongation of the action potential in failing heart. I(to2) density abnormality may cause cardiac arrhythmia and a delayed after-depolarization. Impaired Ca(2+) handing in HF cells rather than reduced CLCA function itself may result in this abnormality.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Fisiologia / Cálcio / Canais de Cloreto / Técnicas de Patch-Clamp / Remodelação Ventricular / Canais de Cálcio Tipo L / Insuficiência Cardíaca Limite: Animais Idioma: Chinês Revista: Chinese Journal of Cardiology Ano de publicação: 2006 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Fisiologia / Cálcio / Canais de Cloreto / Técnicas de Patch-Clamp / Remodelação Ventricular / Canais de Cálcio Tipo L / Insuficiência Cardíaca Limite: Animais Idioma: Chinês Revista: Chinese Journal of Cardiology Ano de publicação: 2006 Tipo de documento: Artigo