Determination of taxifolin in Polygonum orientale of different storage period / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2779-2781, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-238646
ABSTRACT
<p><b>OBJECTIVE</b>To find out the correlation between the content of taxifolin in Polygonum orientale and the storage time.</p><p><b>METHOD</b>HPLC was used to determine taxifolin. The chromatographic condition was as following Diamonsil C18 column (4.6 mm x 200 mm, 5 microm), mobile phase acetonitrile -0.1% phosphoric acid (gradient elution), the detection wavelength 290 nm and flow rate 1.0 mL x min(-1), the column temperature 30 degrees C.</p><p><b>RESULT</b>The injection volume of taxifolin was in good linearity within 0.07 and 0.35 microg, the average recovery was 99.7% with RSD 0.2%. Taxifolin content was 0.84, 1.36, 1.75, 1.99 mg x g(-1) corresponding to storage time of 10, 7, 6, 5 years, respectively.</p><p><b>CONCLUSION</b>The content of taxifolin decreased with the storage time. When the storage period is more than six years, the content is lower than that required by Chinese Pharmacopoeia (2010 version). This method has a good repeatability and accuracy, it provides a scientific reference for clinical use and quality evaluation of P. orientale.</p>
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Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Quercetina
/
Medicamentos de Ervas Chinesas
/
Química
/
Cromatografia Líquida de Alta Pressão
/
Polygonum
/
Estabilidade de Medicamentos
/
Armazenamento de Medicamentos
/
Métodos
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2013
Tipo de documento:
Artigo
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