Effect of Anti-KDR Antibody on the Proliferation of Hemangioma Vascular Endothelial Cells in vitro / 华中科技大学学报（医学）（英德文版）

ABSTRACT

The suppressive effect of anti-KDR antibody against VEGF on proliferation of beman-gioma-derived vascular endothelial cells (HVECs) was investigated. HVECs from one case of he-mangioma in proliferative phase were cultured. Both primary culture and sub-culture were conducted in M199 medium The HVECs of passage 3 were divided into 4 groups based on the concentrations of anti-KDR antibody. Cell count was performed and inhibitory rate of I-IVECs was measured before and 9 days after interference. The results showed that the number of HVECs in the anti-KDR anti-body-treated groups was significantly decreased and the inhibitory rate of HVECs by anti-KDR anti-body (50, 10 and 2 μg/mL) was 84%, 63% and 39% respectively at 9th day after interference, with the difference being significant. In the control group, the number of HVECs was increased signifi- cantly. In was concluded that the anti-KDR antibody could suppress the activity of VEGF through blocking the KDR, indicating the potential clinical applications of anti-KDR antibody in the treat-ment of hemangioma.