The effect of rosiglitazone on the activity of STAT1 in rats with severe acute pancreatitis / 中华外科杂志
Chinese Journal of Surgery
;
(12): 218-221, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-238922
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effect of rosiglitazone on the activity of signal transducer and activator of transcription 1 in rats with severe acute pancreatitis.</p><p><b>METHODS</b>Fifty-four male Wistar rats were randomly allocated into three groups (n = 18). SO group sham-operated animals served as control, operation was executed and sodium chloride but not sodium taurocholate was injected. SAP group SAP was induced by the retrograde injection of 5% sodium taurocholate into the biliopancreatic duct. ROSI group same as SAP group, but rosiglitazone (6 mg/kg) was administered intravenously 30 min before operation. Rats in each group were sacrificed at 3,6 and 12 h after operation. The levels of serum amylase and histologic scores of pancreatic tissue were measured. The expression of TNF-alpha mRNA in pancreatic tissue were determined by reverse transcription-polymerase chain reaction (RT-PCR). The expression of phosphorylated STAT1 in pancreatic tissue was assayed by immunohistochemistry.</p><p><b>RESULTS</b>Compared to SO group, the levels of serum amylase and phosphorylated STAT1, TNF-alpha mRNA and histologic scores of pancreatic tissue were significantly elevated at the same time points after SAP (P < 0.01). The levels of these detection in ROSI group were lower than those of the SAP group at the same time points (P < 0.05), but higher than SO group (P < 0.05).</p><p><b>CONCLUSIONS</b>STAT1 was activated in severe acute pancreatitis. Rosiglitazone has a protective effects in rats with severe acute pancreatitis. The mechanism of its protective effects maybe that it inhibits the activation of JAK/STAT pathway, which can down-regulate the expression of TNF-alpha mRNA and block the the inflammatory cascade partially.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Pâncreas
/
Pancreatite
/
Patologia
/
Farmacologia
/
Distribuição Aleatória
/
Doença Aguda
/
Ratos Wistar
/
Tiazolidinedionas
/
Modelos Animais de Doenças
/
Tratamento Farmacológico
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Surgery
Ano de publicação:
2009
Tipo de documento:
Artigo
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