Establishment of a hydrogel chip for high-throughput detection of Y chromosome microdeletions / 中华男科学杂志
National Journal of Andrology
;
(12): 109-114, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-238979
ABSTRACT
<p><b>OBJECTIVE</b>To establish a high-sensitivity, high-specificity and low-cost hydrogel chip platform for the clinical screening of Y chromosome microdeletions.</p><p><b>METHODS</b>Site-specific extended primers with a common sequence at the 5' end were used for hybridizing with the target. The Cy5-dUTP was incorporated into the products by primer extension, and the products were labeled with fluorescence. Then the extended products were added to the chip for hybridizing with acrylamide-modified common probes immobilized on the chip. After removal of the free Cy5-dUTP by electrophoresis, the signals were obtained by fluorescence scanning. And the detecting conditions of this method were optimized.</p><p><b>RESULTS</b>SY254 of 9 samples was successfully detected with the hydrogel chip. The results showed that 3 were normal and the other 6 with microdeletions (1 female sample as a negative control), which coincided with the results of conventional multiplex PCR-electrophoresis.</p><p><b>CONCLUSION</b>The hydrogel chip platform we established has provided a new technique for the detection of Y chromosome microdeletions, and is beneficial to the diagnosis and treatment of male infertility.</p>
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Índice:
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Assunto principal:
Aberrações dos Cromossomos Sexuais
/
Carbocianinas
/
Deleção Cromossômica
/
Hidrogéis
/
Análise de Sequência com Séries de Oligonucleotídeos
/
Cromossomos Humanos Y
/
Nucleotídeos de Desoxiuracil
/
Diagnóstico
/
Transtornos do Cromossomo Sexual no Desenvolvimento Sexual
/
Genética
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
/
Masculino
Idioma:
Chinês
Revista:
National Journal of Andrology
Ano de publicação:
2012
Tipo de documento:
Artigo
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