Differential expression of miRNAs in myocardial tissues of rats with lipopolysaccharide- induced endotoxemia / 南方医科大学学报
Journal of Southern Medical University
;
(12): 213-217, 2015.
Artigo
em Chinês
| WPRIM
| ID: wpr-239210
ABSTRACT
<p><b>OBJECTIVE</b>To analyze the differential miRNA expression profile in the myocardium of rats with lipopolysaccharide (LPS)-induced endotoxemia and explore the role of miRNA in endotoxin-induced myocardial injury.</p><p><b>METHODS</b>Twenty male SD rats received intraperitoneal injection of 10 mg/kg LPS (n=10) or an equivalent amount of saline solution (n=10). At 24 h after LPS injection, the rats were sacrificed to detect myocardial expressions of TLR4, TNF-α and IL-1β using real-time PCR and for observing myocardial ultrastructures under transmission electron microscopy. The differentially expressed miRNA in the myocardium were detected using a miRNA array, and the common differentially expressed miRNAs were selected for verifying their actual expressions using real-time PCR.</p><p><b>RESULTS</b>TLR4, TNF-α and IL-1β were over-activated in the myocardium of LPS-treated rats, in which mitochondria swelling, structural damaged and cytoplasmic vacuoles were observed. In LPS-challenged rats, miR-194-3p, miR-344a-3p, miR-465-3p, miR-501-5p, miR-3596c, miR-185-3p, and miR-877 were found up-regulated significantly, whereas miR-208b-3p, miR-547-3p, miR-141-3p, miR-28-5p, and miR-3585-5p down-regulated in the myocardium.</p><p><b>CONCLUSION</b>Significant differential expression of the miRNAs occurs in the myocardium of LPS-treated rats, suggesting their involvement in endotoxin-induced myocardial injury.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Regulação para Cima
/
Lipopolissacarídeos
/
Fator de Necrose Tumoral alfa
/
Ratos Sprague-Dawley
/
Endotoxemia
/
MicroRNAs
/
Receptor 4 Toll-Like
/
Interleucina-1beta
/
Reação em Cadeia da Polimerase em Tempo Real
/
Injeções Intraperitoneais
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2015
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS