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Effects of HCV core protein on the expression of hypoxia-inducible factor 1 alpha and vascular endothelial growth factor / 中华肝脏病杂志
Chinese Journal of Hepatology ; (12): 751-754, 2011.
Artigo em Chinês | WPRIM | ID: wpr-239334
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of core protein of hepatitis C virus (HCV) on the expression of hypoxia-inducible factor-1alpha (HIF-1alpha) and vascular endothelial growth factor (VEGF).</p><p><b>METHODS</b>Huh7.5.1 cells were transfected with plasmid flag2B-core carrying HCV core gene, expression of HIF-1alpha and VEGF were measured by reverse transcription-polymorphism chain reaction (RT-PCR) and western blot. Enzyme link immunoabsorbent assay (ELISA) were used to detect the level of VEGF in the supernatants.</p><p><b>RESULTS</b>The expression of HIF-1alpha and VEGF mRNA and protein were upregulated after flag2B-core was transfected into Huh7.5.1 cells, and VEGF level in the supernatant was significant elevated as compared to controls [(654.5+/-43.7) pg/ml vs (365.9+/-26.8) pg/ml, t = 653.1%, P less than 0.01]. The expression of HIF-1alpha and VEGF mRNA and protein were downregulated after flag2B-core and HIF-1alpha siRNA were co-transfected into Huh7.5.1 cells, and VEGF level in the supernatant was significantly reduced as compared to controls [(389.2+/-29.6) pg/ml vs (768.8+/-47.3)pg/ml, t = 1330.22, P less than 0.01].</p><p><b>CONCLUSIONS</b>HCV core protein enhances the expression of HIF-1alpha and VEGF. HCV may regulate the expression of HIF-1alpha and VEGF via the core protein.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Transfecção / Proteínas do Core Viral / RNA Interferente Pequeno / Linhagem Celular Tumoral / Fator A de Crescimento do Endotélio Vascular / Subunidade alfa do Fator 1 Induzível por Hipóxia / Genética / Metabolismo Limite: Humanos Idioma: Chinês Revista: Chinese Journal of Hepatology Ano de publicação: 2011 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Transfecção / Proteínas do Core Viral / RNA Interferente Pequeno / Linhagem Celular Tumoral / Fator A de Crescimento do Endotélio Vascular / Subunidade alfa do Fator 1 Induzível por Hipóxia / Genética / Metabolismo Limite: Humanos Idioma: Chinês Revista: Chinese Journal of Hepatology Ano de publicação: 2011 Tipo de documento: Artigo