Expression and significance of miR-125a and Mcl-1 in intestinal tissue after massive small bowel resection in rat / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery
; (12): 495-498, 2014.
Article
em Zh
| WPRIM
| ID: wpr-239370
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression and significance of miR-125a and anti-apoptotic protein Mcl-1 in intestinal tissue after massive small bowel resection in intestinal adaptation.</p><p><b>METHODS</b>Sprague-Dawley rats (54 male rats, 8-week old) were divided into 3 groups randomly, including two control groups. Rats in the experiment group were subjected to 70% massive small bowel resection. Rats in the resection group underwent simple intestinal resection and anastomosis. Rats in the control group underwent laparotomy alone. A 5 cm intestine approximately 1 cm distal to the anastomosis was harvested a week after operation. Expression of Mcl-1 was assessed by immunohistochemistry and real-time PCR was used to detect the expression of miR-125a in intestinal tissue.</p><p><b>RESULTS</b>The positive expression of Mcl-1 in the experiment group was 18.8%(3/16), significantly lower than that in the control group(76.5%, 13/17) and the resection group (83.33%, 15/18)(both P<0.01). The expression of miR-125a in the experiment group was 1.92, significantly higher than that in the control group (1.01) and the resection group (1.05)(both P<0.01).</p><p><b>CONCLUSION</b>miR-125a and anti-apoptotic protein Mcl-1 may play an important role in intestinal adaptation process and they may regulate each other through a certain pathway.</p>
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1
Índice:
WPRIM
Assunto principal:
Síndrome do Intestino Curto
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Cirurgia Geral
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Anastomose Cirúrgica
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Ratos Sprague-Dawley
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MicroRNAs
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Modelos Animais de Doenças
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Proteína de Sequência 1 de Leucemia de Células Mieloides
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Intestino Delgado
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Metabolismo
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Gastrointestinal Surgery
Ano de publicação:
2014
Tipo de documento:
Article