The simultaneous determination of laetrile, paeoniflorin and paeonol in Jingzhi Guizhi Fuling capsule by HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1252-1254, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-239710
ABSTRACT
<p><b>OBJECTIVE</b>To establish an HPLC method for the simultaneous determination of three major bioactive components in Jingzhi Guizhi Fuling capsules namely laetrile, paeoniflorin and paeonol.</p><p><b>METHOD</b>A LiChrospher C18 column (4.6 mm x 250 mm, 5 microm) was used. The chromatography was carried out with a stepwise gradient programming. The mobile phase was acetonitrile-water (containing 0.1% phosphorous acid) and the flow rate was 1.0 mL x min.</p><p><b>RESULT</b>The linear range of laetrile was 12.87-102.94 micron x mL(-1), r = 0.999 9, paeoniflorin 24.84 - 198.7 microg x mL(-1), r = 0.9999 and paeonol 12.57-100.56 microg x mL(-1), r = 0.999 9. The method is accurate with variation less than 1.5 % and recovery more than 95 %.</p><p><b>CONCLUSION</b>The method was successfully applied to analyze three major bioactive components in Jingzhi Guizhi Fuling capsules.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Acetofenonas
/
Plantas Medicinais
/
Benzoatos
/
Hidrocarbonetos Aromáticos com Pontes
/
Cápsulas
/
Medicamentos de Ervas Chinesas
/
Química
/
Reprodutibilidade dos Testes
/
Cromatografia Líquida de Alta Pressão
/
Polyporales
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2005
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS