Expression of JAK2V617F and MPLW515L/K mutation in 30 suspected cases of early myeloproliferative disorders / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 611-614, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-239973
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the prevalence of JAK2V617F and MPLW515L/K mutation in patients with slightly elevated platelets (BPC) or hemoglobin (Hb) not meeting the criteria of polycythemia vera (PV) or essential thrombocythemia (ET).</p><p><b>METHODS</b>Genomic DNA from bone marrow or blood mononuclear cells was screened with allele specific polymerase chain reaction (AS-PCR) for JAK2V617F and MPLW515L/K mutation. The history of thrombosis was assessed retrospectively by patients files.</p><p><b>RESULTS</b>Of 30 patients, 14 (46.7%) were positive for the JAK2V617F mutation, none of them had the MPLW515L/ K. Five of these 14 patients had a history of thrombosis. Follow-up results were available in 22 patients. Among them, 12 patients with JAK2V617F mutation turned out to be MPD in 6-24 months; only 2 out of 10 patients without this mutation evolved to MPD.</p><p><b>CONCLUSION</b>JAK2V617F mutation could be one of the diagnosis criteria of early MPD. No MPLW515L/K expression was found in early MPD.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Seguimentos
/
Diagnóstico Precoce
/
Diagnóstico
/
Janus Quinase 2
/
Receptores de Trombopoetina
/
Genética
/
Metabolismo
/
Mutação
/
Transtornos Mieloproliferativos
Tipo de estudo:
Estudo diagnóstico
/
Estudo observacional
/
Estudo prognóstico
/
Fatores de risco
/
Estudo de rastreamento
Limite:
Adolescente
/
Adulto
/
Idoso
/
Aged80
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Hematology
Ano de publicação:
2008
Tipo de documento:
Artigo
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