Improving isoprene production by engineered heterologous mevalonate pathway in Escherichia coli / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1073-1081, 2015.
Artigo
em Chinês
| WPRIM
| ID: wpr-240595
ABSTRACT
Isoprene is an important precursor of synthetic rubber material. In our previous study, metabolic engineered Escherichia coli strain (BW-01) was constructed and used to produce isoprene. Based on the theory of protein budget, using synthetic biology strategies including the increased copy number of genes and rare codons, we regulated the expression of key enzyme to improve isoprene production in Escherichia coli strain. Under shake-flask conditions, isoprene productivity of the engineered strain (BW-07) increased by 73% compared with BW-01, reached 761.1 mg/L. It provides a reference for further studies.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Pentanos
/
Microbiologia Industrial
/
Butadienos
/
Dosagem de Genes
/
Hemiterpenos
/
Escherichia coli
/
Biologia Sintética
/
Engenharia Metabólica
/
Genética
/
Metabolismo
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2015
Tipo de documento:
Artigo
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