Purification and characterization of anti-clotting protein component (ACPF-7221) from venom of Agkistrodon acutus / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 2169-2173, 2009.
Artigo
em Inglês
| WPRIM
| ID: wpr-240818
ABSTRACT
<p><b>BACKGROUND</b>Snake venom contains a number of components with different pharmacological and biological activities, especially in cancer therapy, and has increasingly become a research focus. This study was designed to isolate and purify a novel anti-clotting protein component from the venom of Agkistrodon acutus, and to explore its physico-chemical properties and biological activity.</p><p><b>METHODS</b>The venom of Agkistrodon was isolated and purified by ion-exchange chromatography on diethylaminoethyl (DEAE)-Sepharose Fast Flow, molecular sieve filtration through Sephadex G75, SP-Sepharose Fast Flow and molecular sieve filtration through Sephadex G50. We detected the activated partial thromboplastin time (APTT) of the eluant to select the anti-clotting protein component of interest. The molecular weight was determined by sodium dodecyl sulfate-polyacrylamid gel electrphoresis (SDS-PAGE) and liquid chromatography. Its protein content was detected by bicinchoninic acid (BCA).</p><p><b>RESULTS</b>SDS-PAGE vertical gel electrophoresis showed that the anticoagulant factor is a tripolymer composed of three proteins whose molecular weights are 25 KDa, 30 KDa and 50 KDa. The factor contains about 65% percent protein.</p><p><b>CONCLUSIONS</b>A novel anti-clotting protein component was purified by ion-exchange chromatography and molecular sieve filtration from the venom of Agkistrodon acutus and was found to be composed of three kinds of proteins.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas
/
Química
/
Cromatografia Líquida de Alta Pressão
/
Cromatografia por Troca Iônica
/
Agkistrodon
/
Venenos de Crotalídeos
/
Eletroforese em Gel de Poliacrilamida
/
Metabolismo
/
Anticoagulantes
Limite:
Animais
Idioma:
Inglês
Revista:
Chinese Medical Journal
Ano de publicação:
2009
Tipo de documento:
Artigo
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