Effect of wenxin capsule on myocyte apoptosis and expression of Bcl-2, Fas gene protein in rats of experimental myocardial ischemia / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
; (12): 769-771, 2003.
Article
em Zh
| WPRIM
| ID: wpr-240864
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the molecular mechanism of Wenxin Capsule (WXC, an effective Chinese composite drug) in preventing and treating myocardial ischemia of coronary heart disease.</p><p><b>METHODS</b>Rat model of myocardial ischemia was established by subcutaneous multi-point injecting isoproterenol. Effect of WXC on cell apoptosis was observed by transmission electron microscopy and TUNEL method, and its effect on apoptotic related gene Bcl-2 and Fas gene protein expression was observed by immunohistochemical method.</p><p><b>RESULTS</b>Isoproterenol induced myocardial ischemic injury could cause evident cardial cell apoptosis, obvious enhance Fas gene protein expression and mild enhance Bcl-2 gene protein expression. WXC could significantly down-regulate Fas, up-regulate Bcl-2 gene protein expression, significantly inhibit and block the myocardial cell apoptosis.</p><p><b>CONCLUSIONS</b>To inhibit and block the event of cell apoptosis through regulating Bcl-2 and Fas gene protein expression in ischemic myocardium might be one of the mechanisms of WXT in preventing and treating myocardial ischemic injury of coronary heart disease.</p>
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1
Índice:
WPRIM
Assunto principal:
Patologia
/
Farmacologia
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Medicamentos de Ervas Chinesas
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Ratos Wistar
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Isquemia Miocárdica
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Apoptose
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Receptor fas
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Proteínas Proto-Oncogênicas c-bcl-2
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Miócitos Cardíacos
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Combinação de Medicamentos
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Integrated Traditional and Western Medicine
Ano de publicação:
2003
Tipo de documento:
Article