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Etablishment of cartilage degeneration model by IL-1 beta in vitro / 中国骨伤
China Journal of Orthopaedics and Traumatology ; (12): 648-653, 2015.
Artigo em Chinês | WPRIM | ID: wpr-240973
ABSTRACT
<p><b>OBJECTIVE</b>To establish a reliable model for drug screening and therapy by culturing rat femoral head and inducing cartilage degeneration quickly in vitro.</p><p><b>METHODS</b>The femoral heads from the same SD rats of two-month old were divided into control group and experimental group respectively. They were cultured with DMEM medium plus 10% fetal bovine serum or DMEM medium plus 10% fetal bovine serum plus 50 ng/ml IL-1β for three days. Femoral heads were fixed in 4% paraformaldehyde, decalcified, dehydrated, embedded in paraffin and cut into slices. Specimens were stained with Toluidine blue and Safranine O-Fast Green FCF. The protein expression levels of type II collagen, MMP13, Sox9 and ADAMTS5 were analyzed by immunofluorescence.</p><p><b>RESULTS</b>Both the Toluidine blue and Safranine O staining were pale in the margin of femoral heads which were stimulated with IL-1β for three days compared to that in control group. The Fast Green FCF staining was positive at the edge of the femoral head in experimental group, which indicated that cartilage became degenerated. The expression levels of both type H collagen and Sox9 were decreased significantly while the expression levels of MMP13 and ADAMTS5 were increased in experimental group.</p><p><b>CONCLUSION</b>The model of cartilage degeneration is established by culturing and inducing the degeneration of the femoral heads quickly in vitro.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Técnicas In Vitro / Doenças das Cartilagens / Ratos Sprague-Dawley / Colágeno Tipo II / Modelos Animais de Doenças / Metaloproteinase 13 da Matriz / Interleucina-1beta / Fatores de Transcrição SOX9 / Cabeça do Fêmur / Genética Tipo de estudo: Estudo prognóstico Limite: Animais / Humanos / Masculino Idioma: Chinês Revista: China Journal of Orthopaedics and Traumatology Ano de publicação: 2015 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Técnicas In Vitro / Doenças das Cartilagens / Ratos Sprague-Dawley / Colágeno Tipo II / Modelos Animais de Doenças / Metaloproteinase 13 da Matriz / Interleucina-1beta / Fatores de Transcrição SOX9 / Cabeça do Fêmur / Genética Tipo de estudo: Estudo prognóstico Limite: Animais / Humanos / Masculino Idioma: Chinês Revista: China Journal of Orthopaedics and Traumatology Ano de publicação: 2015 Tipo de documento: Artigo