Effects of nanosized titanium dioxide on the reproductive system of male mice / 中华男科学杂志
National Journal of Andrology
;
(12): 517-522, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-241309
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the in vivo effects of nanosized titanium dioxide (TiO2) on the main organs, particularly the reproductive system, of male mice.</p><p><b>METHODS</b>Forty-five male ICR mice aged 6 weeks were equally and randomly divided into 2 experimental groups and a control group, intraperitoneally injected with 200 and 500 mg/kg nanosized TiO2 and equal volume of saline, respectively, every other day for 5 times. One week after drug cessation, we obtained the coefficients of the main organs, serum biochemical parameters and the levels of gonadal hormones (T and E2), analyzed the pathological changes of the main organs by HE staining, observed sperm count, motility and abnormality under the microscope, and detected germ cell apoptosis by TUNEL.</p><p><b>RESULTS</b>Compared with the control, the low-dose (200 mg/kg) group showed no significant changes in the above parameters, while the high-dose (500 mg/kg) group exhibited a decrease in the coefficients of the liver, heart and kidneys, and a significant increase in such serum biochemical parameters as ALT, ALT/AST and BUN (P < 0.05). The high-dose group also showed significantly reduced sperm density and motility, increased sperm abnormality and germ cell apoptosis (P < 0.05), but no obvious pathological changes in the liver, kidney spleen, testis and epididymis.</p><p><b>CONCLUSION</b>Low-dose nanosized TiO2 has no obvious effect on male mice, but high-dose may significantly reduce their sperm count and function and induce germ cell apoptosis, although its damage on the liver and kidney function is slight.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Contagem de Espermatozoides
/
Motilidade dos Espermatozoides
/
Espermatozoides
/
Titânio
/
Apoptose
/
Nanopartículas
/
Camundongos Endogâmicos ICR
Limite:
Animais
Idioma:
Chinês
Revista:
National Journal of Andrology
Ano de publicação:
2009
Tipo de documento:
Artigo
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