Liquid chromatography frontal analysis of the protein binding of glimepiride / 药学学报
Acta Pharmaceutica Sinica
;
(12): 39-42, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-241340
ABSTRACT
<p><b>AIM</b>To study the protein binding of glimepiride.</p><p><b>METHODS</b>An HPLC-FA method is performed by using Pinkerton GFF II-S5-80 internal-surface reversed-phase silica support (150 mm x 4.6 mm ID, 5 microm) at pH 7.4 in a 67 mmol x L(-1) isotonic sodium phosphate buffer at 37 degree C. Other conditions included flow rate of 0.2 mL x min(-1), UV detection at wavelength 230 nm and injection volume 900 microL.</p><p><b>RESULTS</b>Nonlinear regression parameter estimation was used for the association constant measurement of glimepiride to both primary and secondary sites, which were 5.1 (micromol x L(-1)-1 and 1 for K1 and n1, and 0.017 (micromol x L(-1))-1 and 7 for K2 and n2, respectively.</p><p><b>CONCLUSION</b>The method is shown to be suitable for investigation of protein binding of glimepiride.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Ligação Proteica
/
Compostos de Sulfonilureia
/
Albumina Sérica
/
Cromatografia Líquida de Alta Pressão
/
Hipoglicemiantes
/
Metabolismo
/
Métodos
Limite:
Humanos
Idioma:
Chinês
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2005
Tipo de documento:
Artigo
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