Pharmacokinetics of mangiferin in rat plasma after oral administration of a single dose of suanzaoren decoction / 药学学报

ABSTRACT

<p><b>AIM</b>To study the pharmacokinetics of mangiferin in rats after oral administration of a single dose of Suanzaoren decoction.</p><p><b>METHODS</b>An HPLC method was established using puerain as internal standard. Plasma samples were deproteinized with acetonitrile-acetic acid (91), followed by evaporation of the acetonitrile to dryness. The resultant residue was then dissolved in mobile phase and HPLC separation was achieved on a Hypersil C18 (200 mm x 4.6 mm ID, 5 microm) column at room temperature. The mobile phase consisted of acetonitrile-water (1288) with 1% acetic acid and 1% tetrahydrofuran at a flow rate of 0.7 mL x min(-1). The UV detection wavelength was set at 320 nm.</p><p><b>RESULTS</b>The calibration curve was shown to be linear over the range from 0.536 to 26.8 microg x mL(-1) (r2 > or = 0.995). Mean recovery was determined as 92.7%. Within-day and between-day precisions were less than 9. 1% RSD. The limit of quantitation (LOQ) was 0.536 microg x mL(-1). The maximum plasma concentration (Cmax), the time to reach peak concentration (Tmax) and the apparent elimination half-life (T1/2) were (10.5 +/- 2.2) microg x mL(-1), (5.8 +/- 0.4) h and (5.0 +/- 0.3) h, respectively.</p><p><b>CONCLUSION</b>The validated HPLC method developed has been applied to take a limited view of pharmacokinetics profile of mangiferin in rat plasma after having orally taken a single dose of Suanzaoren decoction.</p>