Protective effect of M3 receptor on H2O2 -induced apoptosis of rat myocardial cells in vitro / 药学学报
Acta Pharmaceutica Sinica
;
(12): 887-891, 2004.
Artigo
em Inglês
| WPRIM
| ID: wpr-241417
ABSTRACT
<p><b>AIM</b>To observe the effect of activation of M3 receptor on H2O2 induced apoptosis in cultured rat myocytes and to investigate its possible mechanisms.</p><p><b>METHODS</b>Isolated neonatal cardiomyocytes were cultured. Morphologic changes were observed by microscopy. The apoptosis in cardiomyocyte was detected by terminal deoxynucleotide transferase directed d-UTP nick and end labeling (TUNEL) assay. The expression of apoptosis-related protein in Bcl-2 and Fas was measured by immunohistochemistry assay. [Ca2+]i in single cardiomyocyte loaded with Fluo 3-AM was measured by confocal microscope.</p><p><b>RESULTS</b>H2O2-mediated myocyte apoptosis was attenuated by M3 receptor agonist choline (10 mmol x L(-1)). Pretreatment of cardiac myocytes with choline also increased Bcl-2, decreased Fas expression, and inhibited the increase in FI value of [Ca2+]i in H2O2-stimulated cardiac myocytes. However, blockade of M3 receptor by 4DAMP (10 nmol x L(-1)) completely inhibited the effects of choline on H2O2-stimulated cardiac myocytes.</p><p><b>CONCLUSION</b>Activation of M3 receptor showed protective effect on H2O2-induced apoptosis in cultured rat myocytes and this effect might be related to modulating the expression of some genes including Bcl-2 and Fas as well as the downregulation of [Ca2+]i.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Células Cultivadas
/
Cálcio
/
Colina
/
Ratos Wistar
/
Apoptose
/
Receptor fas
/
Proteínas Proto-Oncogênicas c-bcl-2
/
Biologia Celular
/
Miócitos Cardíacos
Limite:
Animais
Idioma:
Inglês
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2004
Tipo de documento:
Artigo
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