The construction and expression of Saccharomyces albicans pACT1-GFP plasmids / 华西口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To construct strains containing green fluorescent protein (GFP) to study gene regulation in Saccharomyces albicans cells during the infection process.</p><p><b>METHODS</b>pACT1-GFP was constructed, and Saccharomyces albicans CAI4 was transformed. The expression of GFP in yeast and hyphal compartments was observed with microscopy.</p><p><b>RESULTS</b>99% of Saccharomyces albicans cells containing pACT1-GFP fusion displayed significant fluorescence levels both in the yeast and hyphal compartments. The fluorescence intensity in two compartments had no obvious difference.</p><p><b>CONCLUSION</b>pACT1-GFP can be expressed stably in the yeast cells.</p>