Effect of rosiglitazone on the expression of HIF1α and IGF1 mRNA in myeloma / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 641-644, 2014.
Artigo
em Chinês
| WPRIM
| ID: wpr-242097
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of rosiglitazone (RGZ) on the mRNA expression of HIF1α and IGF1 genes in the myeloma cells and explore possible mechanism of angiogenesis inhibition.</p><p><b>METHODS</b>Human myeloma cell line RPMI8226 and primary myeloma cells from five patients enriched by using CD138 immunomagnetic beads were treated with different concentrations (10, 20, 40 μmol/L) of RGZ. The mRNA expression of HIF1α and IGF1 was analyzed in cells treated with RGZ after 48h by RT-PCR, The levels of phosphorylated AKT and ERK proteins were detected by Western blotting. Bone marrow mononuclear cells from five patients with iron deficiency anemia were regarded as control.</p><p><b>RESULTS</b>Higher mRNA expression of HIF1α and IGF1 genes in RPMI8226 and in primary myeloma cells was showed as compared to those in control. Treated with RGZ of 20 μmol/L after 48 h, the mRNA expression of HIF1α (1.21 ± 0.08 vs 0.75 ± 0.06) and IGF1 (0.62 ± 0.06 vs 0.32 ± 0.04) in RPMI8226 cells was declined as compared to those without RGC treatment. The same declination was also seen in primary myeloma cells (HIF1α 2.02 ± 0.16 vs 0.53 ± 0.04; IGF1 1.92 ± 0.13 vs 0.58±0.03). RGZ could inhibit the expression of pAKT and pERK, nor the total AKT and ERK proteins, in RPMI8226 cells in a dose-dependent manner at the concentration of 10 μmol/L, 20 μmol/L, and 40 μmol/L.</p><p><b>CONCLUSION</b>RGZ could inhibit the mRNA expression of HIF1α and IGF1. Inhibition of angiogenesis by RGZ may be associated with down-regulation of pAKT and pERK expression.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
RNA Mensageiro
/
Fator de Crescimento Insulin-Like I
/
Regulação Neoplásica da Expressão Gênica
/
Linhagem Celular Tumoral
/
Tiazolidinedionas
/
MAP Quinases Reguladas por Sinal Extracelular
/
Proteínas Proto-Oncogênicas c-akt
/
Subunidade alfa do Fator 1 Induzível por Hipóxia
/
Genética
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Hematology
Ano de publicação:
2014
Tipo de documento:
Artigo
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