Cloning and application of a novel hydroxylase in lovastatin conversion / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1590-1598, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-242434
ABSTRACT
Wuxistatin, a novel and potent statin, is converted from lovastatin by Amycolatopsis sp. CGMCC1149. In the bioconversion, lovastatin is firstly hydroxylated by a hydroxylase. To obtain the critical hydroxylase, a novel hydroxylase gene was isolated from Amycolatopsis sp. CGMCC1149 by Degenerate PCR and Self-Formed Adaptor PCR and expressed in Escherichia coli. BLAST sequence analysis revealed that the gene belonged to cytochrome P450 gene superfamily and could encode a 403-amino-acid protein with a molecular weight of 44.8 kDa. The secondary structure prediction result showed that this protein contained many typical functional regions of P450, such as oxygen binding site, ion-pair region and heme binding region. Meanwhile, a catalytic function verification system was constructed by NADH, ferredoxin and ferredoxin reductase which could catalyze lovastatin hydroxylation into the target product. These would be helpful for further studies in large-scale production of wuxistatin.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Microbiologia Industrial
/
Actinomycetales
/
Butiratos
/
Lovastatina
/
Dados de Sequência Molecular
/
Sequência de Aminoácidos
/
Clonagem Molecular
/
Sistema Enzimático do Citocromo P-450
/
Genética
/
Hidroxilação
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2013
Tipo de documento:
Artigo
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