Construction and fermentation control of reductive TCA pathway for malic acid production in Saccharomyces cerevisiae / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1484-1493, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-242463
ABSTRACT
Malic acid is widely used in food, and chemical industries. Through overexpressing pyruvate carboxylase and malate dehydrogenase in pdc1-deficient Saccharomyces cerevisiae, malic acid was successfully produced through the reductive TCA pathway. No malic acid was detected in wild type Saccharomyces cerevisiae, however, 45 mmol/L malic acid was produced in engineered strain, and the concentration of byproduct ethanol also reduced by 18%. The production of malic acid enhanced 6% by increasing the concentration of Ca2+. In addition, the final concentration reached 52.5 mmol/L malic acid by addition of biotin. The increasing is almost 16% higher than that of the original strain.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Oxirredução
/
Piruvato Carboxilase
/
Saccharomyces cerevisiae
/
Microbiologia Industrial
/
Transdução de Sinais
/
Ciclo do Ácido Cítrico
/
Redes e Vias Metabólicas
/
Fermentação
/
Engenharia Metabólica
/
Genética
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2013
Tipo de documento:
Artigo
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