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Engineering Saccharomyces cerevisiae for sclareol production / 生物工程学报
Chinese Journal of Biotechnology ; (12): 1185-1192, 2013.
Artigo em Chinês | WPRIM | ID: wpr-242490
ABSTRACT
Sclareol is a member of labdane type diterpenes mostly used as fragrance ingredient. To enable microbial production of sclareol, synthetic pathways were constructed by incorporating labdenediol diphosphate synthase (LPPS) and terpene synthase (TPS) of the plant Salvia sclarea into Saccharomyces cerevisiae. It was found that sclareol production could be benefited by overexpression of key enzyme for precursor biosynthesis, construction of fusion protein for substrate channeling, and removal of signal peptides from LPPS and TPS. Under optimal shake flask culture conditions, strain S6 produced 8.96 mg/L sclareol. These results provided useful information for development of heterologous hosts for production of terpenoids.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Saccharomyces cerevisiae / Proteínas Recombinantes de Fusão / Química / Alquil e Aril Transferases / Salvia / Diterpenos / Redes e Vias Metabólicas / Engenharia Metabólica / Genética / Metabolismo Idioma: Chinês Revista: Chinese Journal of Biotechnology Ano de publicação: 2013 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Saccharomyces cerevisiae / Proteínas Recombinantes de Fusão / Química / Alquil e Aril Transferases / Salvia / Diterpenos / Redes e Vias Metabólicas / Engenharia Metabólica / Genética / Metabolismo Idioma: Chinês Revista: Chinese Journal of Biotechnology Ano de publicação: 2013 Tipo de documento: Artigo