GATA3 siRNA inhibits the binding of NFAT1 to interleukin-13 promoter in human T cells / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 739-744, 2010.
Artigo
em Inglês
| WPRIM
| ID: wpr-242579
ABSTRACT
<p><b>BACKGROUND</b>Interleukin-13 (IL-13) is recognized to be a key modulator in the pathogenesis of Th2-induced allergic inflammation. Transcription factors GATA3 and NFAT1 have been both implicated in the regulation of Th2 cytokines. We previously demonstrated the GATA3-NFAT1 association during human T cell activation. However, the function of the GATA3-NFAT1 complex in Th2 cytokines regulation is still unknown. Small interference RNA (siRNA) was constructed to knock down GATA3 expression in Hut-78 cells to investigate the possible role of GATA3-NFAT1 complex in IL-13 transcription.</p><p><b>METHODS</b>Cells were stimulated with anti-CD3 plus anti-CD28 antibodies to mimic in vivo antigen-mediated co-stimulation; the expression of IL-13 mRNA was determined by real-time PCR; chromation immunoprecipitation (CHIP) assay was employed to investigate the NFAT1 binding to IL-13 promoter.</p><p><b>RESULTS</b>GATA3 siRNA suppressed the expression of GATA3 both in mRNA and protein levels in Hut-78 cells. The binding of NFAT1 to IL-13 promoter was inhibited by GATA3 siRNA in activated T cells, which was followed by the reduction of IL-13 transcription.</p><p><b>CONCLUSION</b>GATA3-NFAT1 complex may play an important role in the regulation of IL-13 transcription in human T cells.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Linfócitos T
/
Transfecção
/
Células Cultivadas
/
Regiões Promotoras Genéticas
/
Interleucina-13
/
RNA Interferente Pequeno
/
Fator de Transcrição GATA3
/
Fatores de Transcrição NFATC
/
Genética
/
Metabolismo
Limite:
Humanos
Idioma:
Inglês
Revista:
Chinese Medical Journal
Ano de publicação:
2010
Tipo de documento:
Artigo
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