Multiplex PCR assay for dissemination and diversity of extended-spectrum beta-lactamase genes in Shigella isolates / 中华预防医学杂志
Chinese Journal of Preventive Medicine
;
(12): 201-205, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-242667
ABSTRACT
<p><b>OBJECTIVE</b>To develop a rapid and simple multiplex polymerase chain reaction (PCR) method which discriminates extended-spectrum beta-lactamases (ESBLs) genes in sporadic Shigella isolates from 1998 to 2007 in Hangzhou city, China.</p><p><b>METHODS</b>After ESBLs screening according to the Clinical and Laboratory Standards Institute (CLSI) method, CTX-M, TEM, SHV and OXA-1 encoding genes were detected by using a multiplex PCR method, and the results were verified by 8 single gene PCR amplification.</p><p><b>RESULTS</b>Seventeen isolates harbored ESBLs genes among 195 Shigella isolates (8.72%). Genes encoding CTX-M (17 strains), TEM (2 strains), OXA-1 (10 strains) and SHV (0 strains) were discriminated with multiplex PCR analysis, which coincided with eight single gene PCR analysis at 94.12%.</p><p><b>CONCLUSION</b>Multiplex PCR should be a suitable tool for initial rapid screening and discriminating ESBLs genes in Shigella isolates. With similar trend of national surveillance data, the proportion of sporadic Shigella isolates harbouring ESBLs genes might probably be on increase.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Shigella
/
Beta-Lactamases
/
DNA Bacteriano
/
Testes de Sensibilidade Microbiana
/
Reação em Cadeia da Polimerase
/
Genes Bacterianos
/
Genética
/
Genótipo
/
Métodos
Tipo de estudo:
Guia de Prática Clínica
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Preventive Medicine
Ano de publicação:
2009
Tipo de documento:
Artigo
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