Application of pulsed-field gel electrophoresis in a food-borne outbreak of Salmonella serotype Muenchen infection / 中华预防医学杂志
Chinese Journal of Preventive Medicine
;
(12): 827-830, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-242708
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the application of pulsed-field gel electrophoresis (PFGE) in food-borne outbreak.</p><p><b>METHODS</b>Pathogens were isolated and further characteristics identified by traditional methods. The strains isolated were carried out with molecular typing with using PFGE. PFGE was performed by Laboratory Directions for molecular subtyping of Salmonella by PFGE (CDC, USA) and the results of PFGE were analyzed by BioNumerics soft.</p><p><b>RESULTS</b>Totally 14 Salmonella serotype Muenchen strains were isolated from 19 patients, 3 of 9 suspicious foods were positive for S. muenchen and 7 strains were isolated from 18 cooks. The biochemistry characterization and antimicrobial susceptibility of all the strains isolated were the same. 23 S. muenchen isolates were all shown indistinguishable by PFGE.</p><p><b>CONCLUSION</b>PFGE should play a key role in identifying the outbreak-associated isolates and distinguishing them from unrelated sporadic isolates. It might also demonstrate that the genetic fingerprints of serotype Muenchen isolates derived from patients were indistinguishable from those derived from drinks. PFGE might provide precise information on bacterial food-borne pathogens, promptly identify the source of infection, and effectively prevent from spreading. It should be one of the early warning method on controlling outbreak of the food-borne disease.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Intoxicação Alimentar por Salmonella
/
Testes de Sensibilidade Microbiana
/
Sorotipagem
/
China
/
Epidemiologia
/
Surtos de Doenças
/
Classificação
/
Eletroforese em Gel de Campo Pulsado
/
Salmonella enterica
/
Métodos
Limite:
Humanos
País/Região como assunto:
Ásia
Idioma:
Chinês
Revista:
Chinese Journal of Preventive Medicine
Ano de publicação:
2008
Tipo de documento:
Artigo
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