The Influence of Diabetes on of PGE2, MMP-14 and TIMP Expressions in Human Chronic Periodontitis / 대한치주과학회지
The Journal of the Korean Academy of Periodontology
; : 755-766, 2007.
Article
em Ko
| WPRIM
| ID: wpr-24278
Biblioteca responsável:
WPRO
ABSTRACT
PURPOSE: The purposes of this study were to compare and quantify the expression of PGE2, MMP-14 and TIMP-1 in the gingival tissues of patients with type 2 diabetes mellitus and healthy adults of chronic periodontitis with alveolar bone resorption. MATERIALS AND METHODS: Gingival tissue samples were obtained during periodontal surgery or tooth extraction. According to the patient's systemic condition & clinical criteria of gingiva, each gingival sample was devided into three groups. Group 1 (n=8) is clinically healthy gingiva without bleeding and no evidence of bone resorption or periodontal pockets, obtained from systemically healthy 8 patients. Group 2 (n=8) is inflammed gingiva from patients of chronic periodontitis with alveolar bone resorption. Group 3 (n=8) is inflammed gingiva from patients of chronic periodontitis with alveolar bone resorption associated with type 2 diabetes. Tissue samples were prepared and analyzed by Western blotting. The quantification of PGE2, MMP-14 and TIMP-1 were performed using a densitometer and statistically analyzed by one-way ANOVA followed by Tukey test. RESULTS: The expressions of MMP-14 and TIMP-1 were showed increasing tendency in group 2 & 3 compared to group 1. The expressions of PGE2, MMP-14 were showed increasing tendency in group 3 compared to group 1 and group 2. According to MMP-14 levels were increasing, PGE2 showed increasing tendency in group 3, and although PGE2, MMP-14 levels were increasing, TIMP-1 levels were similar expressed comparing to group 2. CONCLUSION: In conclusion, this study demonstrated that the expression levels of MMP-14 and TIMP-1 had increasing tendency in inflammed tissue. It can be assumed that PGE2 and MMP-14 may be partly involved in alveolar bone resorptive process and the progression of periodontal inflammation associated to type 2 DM.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Bolsa Periodontal
/
Extração Dentária
/
Reabsorção Óssea
/
Dinoprostona
/
Western Blotting
/
Inibidor Tecidual de Metaloproteinase-1
/
Diabetes Mellitus Tipo 2
/
Periodontite Crônica
/
Gengiva
/
Hemorragia
Limite:
Adult
/
Humans
Idioma:
Ko
Revista:
The Journal of the Korean Academy of Periodontology
Ano de publicação:
2007
Tipo de documento:
Article