False human immunodeficiency virus test results associated with rheumatoid factors in rheumatoid arthritis / 中国医学科学杂志(英文版)
Chinese Medical Sciences Journal
;
(4): 103-106, 2014.
Artigo
em Inglês
| WPRIM
| ID: wpr-242890
ABSTRACT
<p><b>OBJECTIVE</b>To investigate if immunological factors associated with rheumatoid arthritis (RA) affect the result of human immunodeficiency virus (HIV) screening by electrochemiluminescence immunoassay (ECLIA) and enzyme-linked immunosorbent assay (ELISA).</p><p><b>METHODS</b>100 RA cases were enrolled from January 2012 to February 2013 into this study. HIV screening was conducted with ECLIA detecting both HIV-1 p24 antigen, HIV-1 and HIV-2 antibodies, with ELISA and colloidal gold method detecting HIV-1 and HIV-2 antibodies. The samples producing positive results were submitted to the Center for Disease Control for confirmation using Western blotting method. The antibody titers of rheumatoid factors (RF) including RF-IgG, RF-IgM, RF-IgA, and CCP-IgG were analyzed by ELISA.</p><p><b>RESULTS</b>The HIV positive-rate determined by ECLIA was significantly higher than that by ELISA and colloidal gold method (P<0.01). The false-positive rate of HIV screening was associated with antibody titers of RF-IgG, RF-IgM, RF-IgA, and CCP-IgG in RA (P<0.01).</p><p><b>CONCLUSIONS</b>Immunological factors, including RF and anti-CCP antibody, may influence the screening of HIV by ECLIA, producing false-positive result.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Artrite Reumatoide
/
Fator Reumatoide
/
Sangue
/
Ensaio de Imunoadsorção Enzimática
/
Anticorpos Anti-HIV
/
Antígenos HIV
/
Infecções por HIV
/
Diagnóstico
/
Reações Falso-Positivas
Tipo de estudo:
Estudo diagnóstico
Limite:
Adulto
/
Idoso
/
Humanos
Idioma:
Inglês
Revista:
Chinese Medical Sciences Journal
Ano de publicação:
2014
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS