Culture and identify the human umbilical vein endothelial cells and investigate the expression of tyrosine kinase-2 with immunoglobulin-like and epidermal growth factor homology domains in the cells / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 653-656, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-242927
ABSTRACT
<p><b>OBJECTIVE</b>To study the cultural method and identification of human umbilical vein endothelial cells (HUVECs), and investigate the expression of tyrosine kinase-2 with immunoglobulin-like and epidermal growth factor homology domains(Tie-2) in HUVECs.</p><p><b>METHODS</b>HUVECs were isolated from umbilical veins by the technique of irrigative digestion, and were cultivated in plates. The cells were identified by VIII monoclonal antibody. Tie-2 mRNA and protein were detected by reverse transcription-polymerase chain reaction (RT-PCR) and SABC immunocytochemistry.</p><p><b>RESULTS</b>HUVECs could adhere to the plates completely after 24 hours, and confluence a monolayer 4-5 days later. The band of Tie-2 mRNA was obviously and the expression of Tie-2 protein was strongly positive by immunocytochemistry in HUVECs. The positive rate was over 85%.</p><p><b>CONCLUSION</b>Highly purified endothelial cells were isolated. And there were overexpression of Tie-2 in HUVECs.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Veias Umbilicais
/
Imunoglobulinas
/
Células Cultivadas
/
Células Endoteliais
/
TYK2 Quinase
/
Células Endoteliais da Veia Umbilical Humana
/
Família de Proteínas EGF
Tipo de estudo:
Estudo prognóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
West China Journal of Stomatology
Ano de publicação:
2009
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS