Mutation analysis of the eda-A1 gene for hypohidrotic ectodermal dysplasia and construction of recombined eukaryotic expression vector / 华西口腔医学杂志
West China Journal of Stomatology
; (6): 610-613, 2009.
Article
em Zh
| WPRIM
| ID: wpr-242938
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>The purpose of this study was to clone and analyze mutation in the eda-A1 gene for hypohidrotic ectodermal dysplasia (HED), and to construct a new recombined eukaryotic expression vector (mutant M, wild W) as a basis for further study on the genetic function.</p><p><b>METHODS</b>After total mRNA was extracted from peripheral blood lymphocytes from the HED affect patient and control, eda-A1 gene was amplified by reverse transcription polymerase chain reaction (RT-PCR) with a pair of specific primers containing the constriction enzyme sites of BamH I and Hind III. When the vector pcDNA3.1(-) and eda-A1 (M/W) were digested by BamH I and Hind III respectively, eda-A1 (M/W) fragment was then ligated to vector pcDNA3.1 (-) and the new vector was named as pcDNA3.1 (-)-eda-A1-M/W.</p><p><b>RESULTS</b>eda-A1 gene was successfully cloned and a novel missence mutation was identified, which changes the codon 306 from glutamine to proline. PCR, restrictive endonuclease analysis and DNA sequencing were then performed to identify the recombinant eukaryotic expression vector pcDNA3.1 (-)-eda-A1-M/W, and the results were surely confirmed.</p><p><b>CONCLUSION</b>Our result indicates that the novel missense mutation in eda is associated with the isolated tooth agenesis and provide preliminary explanation for the abnormal clinical phenotype at a molecular structural level. And also, the recombinant eukaryotic expression vector pcDNA3.1 (-)-eda-A1-M/W was successfully constructed, which will be thereafter taken use of further study on eda gene in odontogenesis.</p>
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Índice:
WPRIM
Assunto principal:
RNA Mensageiro
/
Análise de Sequência de DNA
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Displasia Ectodérmica Anidrótica Tipo 1
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Vetores Genéticos
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Mutação
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Odontogênese
Limite:
Humans
Idioma:
Zh
Revista:
West China Journal of Stomatology
Ano de publicação:
2009
Tipo de documento:
Article