The effect of Nifedipine and mechanical strain on matrix metalloproteinase expression in human periodontal ligament fibroblast / 华西口腔医学杂志
West China Journal of Stomatology
; (6): 366-369, 2009.
Article
em Zh
| WPRIM
| ID: wpr-243002
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of Nifedipine on matrix metalloproteinase (MMP)-10 and MMP-13 expression in human periodontal ligament fibroblast (HPDLF) induced by mechanical strain in vitro.</p><p><b>METHODS</b>HPDLF was divided into four groups at random by mechanical strain of elongation 0%, 8%, 12% and 16%. Each group was divided into four subgroups again by the concentration of Nifedipine 0, 10, 30, 50 microm. The cells were imposed to state mechanical strain 12 h after incubated with Nifedipine 1 h. And then immunohistochemical staining was used to investigate the expressions of MMP-10 and MMP-13 in intracytoplasm.</p><p><b>RESULTS</b>In 0% group, there was no significant difference of MMP-10 and MMP-13 expression in all subgroups (P>0.05). The expressions of MMP-10 and MMP-13 were high in group 8%, 12% and 16% without Nifedipine, and increasing significantly with elongation (P<0.001). The expressions of MMP-10 and MMP-13 decreased with dose increasing (P<0.001) in HPDLF after imposed to Nifidepine.</p><p><b>CONCLUSION</b>The expressions of MMP-10 and MMP-13 induced by mechanical strain were inhibited by Nifedipine, which suggested calcium ions channels participate in expressions of MMP-10 and MMP-13 induced by loading.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Ligamento Periodontal
/
RNA Mensageiro
/
Nifedipino
/
Metaloproteinases da Matriz
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Fibroblastos
Limite:
Humans
Idioma:
Zh
Revista:
West China Journal of Stomatology
Ano de publicação:
2009
Tipo de documento:
Article