Effects of RhoA siRNA on the proliferation, adhesion, migration and invasion of tongue squamous cell carcinoma Tca8113 cells in vitro / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 520-524, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-243151
ABSTRACT
<p><b>OBJECTIVE</b>to investigate the effect of RhoA on the metastasis of tongue squamous cell carcinoma Tca8113 cells in vitro.</p><p><b>METHODS</b>a group of RhoA specific small interfering RNAs (siRNA) was constructed and confined by sequencing analysis. The siRNA of RhoA gene was transfected into human tongue squamous cell carcinoma Tca8113 cells line by Lipofectamine(TM) 2000. The protein transient expression of RhoA in the transfectants was examined 48 h after transfection. The cell line with stable expression of siRNA of RhoA was obtained by blasticidin screening and colony culture. Cell growth rate was determined with a cell counting. Millicell chambermodel and wound healing assay were used to examine the abilities of migration and invasion, respectively in vitro.</p><p><b>RESULTS</b>RhoA was overexpressed in tongue squamous cell carcinoma Tca8113 cell line. Silencing of endogenous RhoA gene expression in Tca8113 cells resulted in inhibition of the proliferation, adhesion, chemotaxis and invasion of Tca8113 cells in vitro.</p><p><b>CONCLUSIONS</b>RhoA siRNA inhibits the proliferation, adhesion, chemotaxis and invasion of oral squamous cell carcinoma Tca8113 cell lines. siRNA of RhoA is a potential factor controlling the proliferation and metastasis of Tca8113 cells. RhoA may play an important role in metastasis of oral squamous cell carcinoma.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Técnicas In Vitro
/
Neoplasias da Língua
/
Carcinoma de Células Escamosas
/
Transfecção
/
Adesão Celular
/
Ciclo Celular
/
Movimento Celular
/
RNA Interferente Pequeno
/
Linhagem Celular Tumoral
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Stomatology
Ano de publicação:
2010
Tipo de documento:
Artigo
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