Effects of Artesunate on hepatic fibrosis and its mechanism / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 14-17, 2015.
Artigo
em Chinês
| WPRIM
| ID: wpr-243445
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of Artesunate(Art) on the LX-2 cell.</p><p><b>METHODS</b>The cultured hepatic stellate cells were divided into control group and Art-treated groups with 250,350,450 µmol/L. The rate of cellular proliferation was detected by MIT assay, the content of ceramide (Cer)was determined by HPLC method, the content of hydroxyproline (Hyp) was determined by enzyme digestion method, the expressions of PPAR-γ, p53 and Caspase 3 were detected by Western blot.</p><p><b>RESULTS</b>Compared with control group, IX-2 treated with Art were inhibited in a concentration-dependent manner(P < 0.01). Art could significantly increase the content of cerarnide in LX-2 ( P <0.01), and the content of Hyp was significantly decreased (P <0.05, P <0.01). The expressions of PPAR-γ, p53 and Caspase 3 were increased compared with that of control group(P < 0.01).</p><p><b>CONCLUSION</b>Artesunate could inhibit the proliferation and induce apoptosis of hepatic stellate cells through upregulating ceramide.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Linhagem Celular
/
Ceramidas
/
Apoptose
/
Artemisininas
/
PPAR gama
/
Proliferação de Células
/
Caspase 3
/
Células Estreladas do Fígado
/
Hidroxiprolina
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2015
Tipo de documento:
Artigo
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