Comparison between immunofluorescence and PCR in detecting human papilloma virus in condyloma acuminata / 中国医学科学杂志(英文版)

ABSTRACT

<p><b>OBJECTIVE</b>To compare the effectiveness of immunofluorescence and polymerase chain reaction (PCR) in detecting human papilloma virus (HPV) in condyloma acuminata (CA).</p><p><b>METHODS</b>HPVs in CA tissues from 60 patients were detected by immunofluorescence and PCR, respectively. Different subtypes of HPVs were also identified with restriction fragment length polymorphism (RFLP).</p><p><b>RESULTS</b>The positive detective rates of immunofluorescence and PCR were 56.67% (34/60) and 96.67% (58/ 60), respectively (P < 0.01). RFLP results showed HPV6 and HPV11 were the main subtypes in the detected virus, which accounted for 98.28%.</p><p><b>CONCLUSION</b>The sensibility of PCR is superior to that of immunofluorescence.</p>