Recent advances in directed evolution / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1-11, 2018.
Artigo
em Chinês
| WPRIM
| ID: wpr-243647
ABSTRACT
Screening is the bottleneck of directed evolution. In order to address this problem, a series of novel semi-rational designed strategies have been developed based on combinatorial active-site saturation test and iterative saturation mutagenesis, including single code saturation mutagenesis, double code saturation mutagenesis and triple code saturation mutagenesis. By creation of "small and smart" high qualified mutant libraries and combinatorial mutagenesis of specific sites, these new strategies have been successfully applied in multiparameter optimization, e.g. stereo/regioselectivity and activity. This review summarized recent advances in directed evolution and its applications in biocatalysis field.
Texto completo:
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Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2018
Tipo de documento:
Artigo
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