Construction of spider silk functional platform via intein trans-splicing / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1704-1714, 2016.
Artigo
em Chinês
| WPRIM
| ID: wpr-243687
ABSTRACT
To provide technical support for spider silk functional modification, we developed a simple and efficient functional platform via intein trans-splicing. Small ubiquitin-related modifier protein (SUMO) was fused to the recombinant spider silk protein (W2CT) by peptide bond via S0 split intein Ssp DnaB trans-splicing, resulting in a protein SUMOW2CT. However, incorporation of exogenous protein led to mechanical property defect and lower fiber yield, and also slowed down the fiber assembly velocity but no obvious differences in supercontraction and chemical resistance when compared with fibers from W2CT (W). SUMO protease digestion showed positive results on the fibers, indicating that the SUMO protein kept its native conformation and bioactive. Above all, this work provides a technical support for spider silk high simply and efficient functionalized modification.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Aranhas
/
Proteínas Recombinantes
/
Química
/
Processamento de Proteína
/
Trans-Splicing
/
Proteínas Modificadoras Pequenas Relacionadas à Ubiquitina
/
Seda
/
Inteínas
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2016
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS