AcSDKP attenuates PDGF-induced cardiac fibroblasts proliferation and collagen expression: role of extracellular regulated protein kinase 1/2 pathway / 中华心血管病杂志
Chinese Journal of Cardiology
; (12): 444-448, 2008.
Article
em Zh
| WPRIM
| ID: wpr-243760
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of AcSDKP on platelet-derived growth factor (PDGF)-induced rat cardiac fibroblasts proliferation and collagen expression and explore the role of extracellular regulated protein kinase 1/2 (ERK1/2) pathway on this process.</p><p><b>METHODS</b>Metabolic activity of fibroblasts was determined by CCK-8. Cell cycle was detected by flow cytometry. Expressions of type I and type III collagen were measured by immunocytochemistry and Western blot. Expressions of phospho-ERK1/2 and ERK1/2 were detected by Western blot.</p><p><b>RESULT</b>10(-9) mol/L AcSDKP could significantly inhibit PDGF-induced cardiac fibroblasts proliferation, collagen expression and expressions of phospho-ERK1/2, while the protein levels of ERK1/2 were not significantly affected by AcSDKP.</p><p><b>CONCLUSION</b>AcSDKP could inhibit PDGF-induced cardiac fibroblasts proliferation and collagen expression through activation of phosphor-ERK1/2 pathway.</p>
Texto completo:
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Índice:
WPRIM
Assunto principal:
Oligopeptídeos
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Fosforilação
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Fator de Crescimento Derivado de Plaquetas
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Transdução de Sinais
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Células Cultivadas
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Colágeno
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Ratos Wistar
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Proteína Quinase 1 Ativada por Mitógeno
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Biologia Celular
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Miócitos Cardíacos
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Cardiology
Ano de publicação:
2008
Tipo de documento:
Article