Study on the potential mechanisms of leukemia cell resistance to TRAIL-induced apoptosis / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 379-382, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-243943
ABSTRACT
<p><b>OBJECTIVE</b>To explore the potential mechanisms of leukemia cell resistance to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) -induced apoptosis.</p><p><b>METHODS</b>Cells apoptosis, changes of mitochondrial membrane potential, activity of NF-kappaB, activity of caspase-8 and expressions of apoptosis-related proteins in TRAIL treated K562 and CEM cells, were detected by flowcytometry, ELISA and Western blotting methods, respectively.</p><p><b>RESULTS</b>After treated with TRAIL, the apoptosis indexes were 29.98% and 14.1%, and mitochondrial membrane potential were decreased to 73.25% and 25.4% in K562 and CEM cells respectively. Constitutive level of caspase-8 expression in CEM was lower than that in K562 cells. Both cells became over-expressed Bcl-xL and down-regulated Bax. The ratio of Bcl-xL/Bax in CEM cells was higher than that in K562 cells. Compared with that in K562, the NF-kappaB activity increased significantly in CEM after treatment with TRAIL in early stage.</p><p><b>CONCLUSION</b>CEM cells were more resistant to TRAIL-induced apoptosis than K562 cells did. The potential mechanisms associated with CEM drug resistance might be the lower expression of the constitutive level of caspase-8, lower sensitivity of mitochondrial inner membrane, early increase in NF-KB activity and altered expression of Bcl-2 proteins family.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Células Tumorais Cultivadas
/
NF-kappa B
/
Apoptose
/
Resistencia a Medicamentos Antineoplásicos
/
Proteínas Proto-Oncogênicas c-bcl-2
/
Células K562
/
Proteínas Reguladoras de Apoptose
/
Caspase 8
/
Ligante Indutor de Apoptose Relacionado a TNF
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Hematology
Ano de publicação:
2006
Tipo de documento:
Artigo
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