Study on mismatch repair genes of chronic myeloid leukemia / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 103-106, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-243986
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression and regulation mechanism of mismatch repair (MMR) genes in chronic myeloid leukemia (CML).</p><p><b>METHODS</b>Expression of MMR genes hMSH2, hMSH3, hMSH6, hMLH1 and hPMS2 mRNAs in 62 CML patients and K562 cell line were detected by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). Expression of bcr-abl mRNA and MMR genes mRNA were detected by RT-PCR in 26 CML patients with allogeneic peripheral blood stem cell transplantation (allo-PBSCT) and 4 CML patients on imatinib treatment. Expression of bcr-abl mRNA was detected by RT-PCR and tyrosine phosphorylation of BCR-ABL fusion protein by Western blot.</p><p><b>RESULTS</b>Expression of hMSH2, hMSH3 and hMLH1 mRNA was significantly lower in CML and K562 cells than in normal control (P < 0.05). In 26 CML with allo-PBSCT and 4 CML patients on imatinib treatment, expressions of hMSH2, hMSH3 and hMLH1 mRNA was enhanced while expression of bcr-abl mRNA decreased. In CML MNC after imatinib treatment and in K562 cells, expression of hMSH2, hMSH3 and hMLH1 mRNA was enhanced while tyrosine phosphorylation of BCR-ABL fusion protein decreased.</p><p><b>CONCLUSION</b>Expressions of hMSH2, hMSH3 and hMLH1 mRNA were down-regulated by bcr-abl fusion gene.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Piperazinas
/
Pirimidinas
/
Benzamidas
/
RNA Mensageiro
/
Leucemia Mielogênica Crônica BCR-ABL Positiva
/
Expressão Gênica
/
Regulação Neoplásica da Expressão Gênica
/
Proteínas de Fusão bcr-abl
/
Células K562
Limite:
Adulto
/
Idoso
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Hematology
Ano de publicação:
2006
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS