A preliminary genetic reassortment between Hantaan virus and Seoul virus strains / 中华流行病学杂志
Chinese Journal of Epidemiology
;
(12): 46-49, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-244302
ABSTRACT
<p><b>OBJECTIVE</b>To determine the frequency and characteristics of reassortment among Hantaan and Seoul viruses causing hemorrhagic fever with renal syndrome (HFRS).</p><p><b>METHODS</b>Mixed infections were initiated in tissue culture, using Hantaan virus strain 76 - 118 and Seoul virus strain SR-11. Potential reassortant virus plaques were picked out by multiplex RT-PCR, using primers specific for individual genome segments (L, M, S) of each strain.</p><p><b>RESULTS</b>Most of the progeny virus plaques (68.19% of 44) had parental genotype of 76 - 118 strain or SR-11 strain while 2 of 44 plaques had mixed genotypes that yielded RT-PCR bands for the same segment of both parental strains. Reassortant viruses were detected in 68.19% of 44 progeny plaques tested, involving the M and S segments. In addition, approximately 4.55% of the progeny virus plaques appeared to contain S or M segments originating from both parental virus strains, showing that they were diploid.</p><p><b>CONCLUSION</b>Genetic reassortment can occur between Hantaan virus and Seoul virus strains.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Células Vero
/
RNA Viral
/
Chlorocebus aethiops
/
Vírus Reordenados
/
Genoma Viral
/
Vírus Hantaan
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Vírus Seoul
/
Genética
/
Genótipo
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Epidemiology
Ano de publicação:
2002
Tipo de documento:
Artigo
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