Reversal of multidrug resistance in renal cell carcinoma by short hairpin RNA targeting MDR1 gene / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 2741-2745, 2012.
Artigo
em Inglês
| WPRIM
| ID: wpr-244363
ABSTRACT
<p><b>BACKGROUND</b>Over-expression of P-glycoprotein (P-gp), encoded by the MDR1 gene, confers multidrug resistance (MDR) in renal cell carcinoma (RCC) and is a major reason for unsuccessful chemotherapy. This study aimed to determine the effct of RNA interference (RNAi) on the reversal of MDR in human RCC.</p><p><b>METHODS</b>We designed and selected one short hairpin RNA (shRNA) targeting MDR1 gene, which is stably expressed from integrated plasmid and transfected by lentivirus fluid in human RCC A498 cell.</p><p><b>RESULTS</b>The MDR1-targeted RNAi resulted in decreased MDR1 gene mRNA level (P < 0.001), almost abolished P-gp expression and reversed MDR to different chemotherapy drugs in the RCC A498 cell line.</p><p><b>CONCLUSION</b>MDR could be reversed by RNAi in human RCC A498 cell line, which may be used for clinical application in future.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Carcinoma de Células Renais
/
Lentivirus
/
Membro 1 da Subfamília B de Cassetes de Ligação de ATP
/
Concentração Inibidora 50
/
RNA Interferente Pequeno
/
Linhagem Celular Tumoral
/
Proliferação de Células
/
Genética
/
Metabolismo
Limite:
Humanos
Idioma:
Inglês
Revista:
Chinese Medical Journal
Ano de publicação:
2012
Tipo de documento:
Artigo
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