Study on identification of Sarcandra glabra and Chloranthus spicatus's leaves by PCR amplification of specific alleles / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 3259-3262, 2014.
Artigo
em Chinês
| WPRIM
| ID: wpr-244585
ABSTRACT
The paper is aimed to identify SNP in Sarcandra glabra and Chloranthus spicatus, and authenticate S. glabra from Ch. spicatus and the mixture by using PCR amplification of specific alleles. SNPs in the ITS sequences of S. glabra and Ch. spicatus were found by ClustulX 2. 1 program and Bioedit software. Primers for authentic S. glabra and Ch. spicatus was designed according to the SNP site, and ITS sequence universal primers plus to the authentic primer to construct a multi-PCR reaction system, and then optimized the PCR reaction system. Five hundred and eighty band special for S. glabra and 470 bp band special for Ch. spicatus were found by using multi-PCR reaction. The multi-PCR reaction system could be applied to identify S. glabra and Ch. spicatus's leaves.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Especificidade da Espécie
/
DNA Ribossômico
/
RNA Ribossômico
/
RNA Ribossômico 18S
/
Reação em Cadeia da Polimerase
/
Classificação
/
Folhas de Planta
/
DNA de Plantas
/
Magnoliopsida
/
Polimorfismo de Nucleotídeo Único
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2014
Tipo de documento:
Artigo
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