The effects of insulin-like growth factor-II on the proliferation of osteoblast-like cells in vitro / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 58-61, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-244831
ABSTRACT
<p><b>OBJECTIVE</b>To seek for the appropriate concentration, at which IGF-II can exerts its strong effects on postirradiation proliferation, physiological function and differentiation of the rat's osteoblast-like cells (ROB).</p><p><b>METHODS</b>The osteoblast-like cells used were isolated from the calvariae of neonatal (one-day-old) SD rats by sequential enzymatic digestion. The third passages of the cells were irradiated with gamma-ray from a (60)Co source at the doses of 100, 400, 600, and 900 cGy. The medium was changed immediately after irradiation and 5 concentrations of IGF-II, i.e., 0, 0.1, 1.0, 10.0, and 100.0 microgram/L were added. 6 days after radiation (9 days in culture), the examination, or the measurement of relative cell number, was carried out.</p><p><b>RESULTS</b>Radiation inhibited the ROB, even lethally. IGF-II completely counteracted the inhibitory effects when the cells were exposed to the radiation at lower dose (100 cGy), and partially when at higher dose (400 cGy). But after the radiation at much higher dose as 900 cGy, the damages were irreversible, even with the existence of this growth factor.</p><p><b>CONCLUSIONS</b>At least a portion of effective recovery of postirradiation damages may be due to IGF-II-induced radioresistance. Incubation with IGF-II can increase radioresistance or repair of radiation-induced cells damages. However, this effect depends on the dose of radiation.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Osteoblastos
/
Farmacologia
/
Fisiologia
/
Efeitos da Radiação
/
Tolerância a Radiação
/
Fator de Crescimento Insulin-Like II
/
Divisão Celular
/
Células Cultivadas
/
Ratos Sprague-Dawley
/
Relação Dose-Resposta a Droga
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Stomatology
Ano de publicação:
2002
Tipo de documento:
Artigo
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