Isolation of mesenchymal stem cells from bone marrow filters by primary explant culture / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 459-463, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-244902
ABSTRACT
This study was aimed to investigate whether mesenchymal stem cells (MSC) can be isolated from bone marrow filters which have always been discarded. The bone marrow (BM) particles from BM filters of 2 healthy donors were cultivated by primary explant culture. After expansion, the number of MSC was counted and their immunophenotype and differentiation potential were detected. The results indicated that many MSC were found in bone marrow particles from filters, and nearly 10(7) MSC were obtained at 3 passages of expansion. They not only possessed the characteristics of morphology and immunophenotype of MSC, but also could differentiate into osteoblasts, chondrocytes and adipocytes. In conclusion, a large amount of MSC can be obtained from BM filters if the BM particles were cultivated by primary explant culture.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Células da Medula Óssea
/
Diferenciação Celular
/
Separação Celular
/
Células Cultivadas
/
Biologia Celular
/
Técnicas de Cultura de Tecidos
/
Células-Tronco Mesenquimais
/
Métodos
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2011
Tipo de documento:
Artigo
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